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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
27/11/2023 |
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Data da última atualização: |
28/11/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SUNIGA, P. A. P.; MANTOVANI, C.; SANTOS, M. G. dos; EGITO, A. A. do; VERBISCK, N. V.; SANTOS, L. R. dos; DÁVILA, A. M. R.; ZIMPEL, C. K.; ZERPA, M. C. S.; CHIEBAO, D. P.; GUIMARÃES, A. M. DE S.; NASSAR, A. F. DE C.; ARAUJO, F. R. |
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Afiliação: |
PAULA ADAS PEREIRA SUNIGA, UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL; CYNTHIA MANTOVANI, CNPGC; MARIA GORETTI DOS SANTOS, CNPGC; ANDREA ALVES DO EGITO, CNPGC; NEWTON VALERIO VERBISCK, CNPGC; LENITA RAMIRES DOS SANTOS, CNPGC; ALBERTO MARTÍN RIVERA DÁVILA, INSTITUTO OSWALDO CRUZ; CRISTINA KRAEMER ZIMPEL, MICHIGAN STATE UNIVERSITY; MARIA CAROLINA SISCO ZERPA, UNIVERSIDASDE DE SÃO PAULO; DANIELA PONTES CHIEBAO, ANIMAL HEALTH RESEARCH CENTER; ANA MÁRCIA DE SÁ GUIMARÃES, UNIVERSIDADE DE SÃO PAULO; ALESSANDRA FIGUEIREDO DE CASTRO NASSAR, ANIMAL HEALTH RESEARCH CENTER; FLABIO RIBEIRO DE ARAUJO, CNPGC. |
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Título: |
Glanders Diagnosis in an Asymptomatic Mare from Brazil: Insights from Serology, Microbiological Culture, Mass Spectrometry, and Genome Sequencing. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Pathogens, v. 12, e1250, 2023. |
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DOI: |
https://doi.org/10.3390/pathogens12101250 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT - This manuscript elucidates the occurrence of glanders in an asymptomatic mare from Brazil presenting positive Burkholderia mallei antibody titers. The diagnosis was established through a multi-pronged approach encompassing microbiological culture, mass spectrometry, and genome sequencing. The outbreak occurred in 2019 in Tatuí, São Paulo, Brazil, and the infected mare, despite displaying no clinical symptoms, had multiple miliary lesions in the liver, as well as intense catarrhal discharge in the trachea. Samples were collected from various organs and subjected to bacterial isolation, molecular detection, and identification. The strain was identified as B. mallei using PCR and confirmed by MALDI-TOF mass spectrometry. Whole-genome sequencing revealed a genome size of 5.51 Mb with a GC content of 65.8%, 5871 genes (including 4 rRNA and 53 tRNA genes), and 5583 coding DNA sequences (CDSs). Additionally, 227 predicted pseudogenes were detected. In silico analysis of different genomic loci that allow for differentiation with Burkholderia pseudomallei confirmed the identity of the isolate as B. mallei, in addition to the characteristic genome size. The BAC 86/19 strain was identified as lineage 3, sublineage 2, which includes other strains from Brazil, India, and Iran. The genome sequencing of this strain provides valuable information that can be used to better understand the pathogen and its epidemiology, as well as to develop diagnostic tools for glanders. |
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Thesagro: |
Doença Animal; Patogenicidade. |
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Thesaurus Nal: |
Animal diseases; Burkholderia mallei; Genome; Glanders; Mass spectrometry; Pathogenicity. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1158739/1/Glanders-diagnosis-Asymptomatic-Mare-2023.pdf
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Marc: |
LEADER 02616naa a2200373 a 4500
001 2158739
005 2023-11-28
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/pathogens12101250$2DOI
100 1 $aSUNIGA, P. A. P.
245 $aGlanders Diagnosis in an Asymptomatic Mare from Brazil$bInsights from Serology, Microbiological Culture, Mass Spectrometry, and Genome Sequencing.$h[electronic resource]
260 $c2023
520 $aABSTRACT - This manuscript elucidates the occurrence of glanders in an asymptomatic mare from Brazil presenting positive Burkholderia mallei antibody titers. The diagnosis was established through a multi-pronged approach encompassing microbiological culture, mass spectrometry, and genome sequencing. The outbreak occurred in 2019 in Tatuí, São Paulo, Brazil, and the infected mare, despite displaying no clinical symptoms, had multiple miliary lesions in the liver, as well as intense catarrhal discharge in the trachea. Samples were collected from various organs and subjected to bacterial isolation, molecular detection, and identification. The strain was identified as B. mallei using PCR and confirmed by MALDI-TOF mass spectrometry. Whole-genome sequencing revealed a genome size of 5.51 Mb with a GC content of 65.8%, 5871 genes (including 4 rRNA and 53 tRNA genes), and 5583 coding DNA sequences (CDSs). Additionally, 227 predicted pseudogenes were detected. In silico analysis of different genomic loci that allow for differentiation with Burkholderia pseudomallei confirmed the identity of the isolate as B. mallei, in addition to the characteristic genome size. The BAC 86/19 strain was identified as lineage 3, sublineage 2, which includes other strains from Brazil, India, and Iran. The genome sequencing of this strain provides valuable information that can be used to better understand the pathogen and its epidemiology, as well as to develop diagnostic tools for glanders.
650 $aAnimal diseases
650 $aBurkholderia mallei
650 $aGenome
650 $aGlanders
650 $aMass spectrometry
650 $aPathogenicity
650 $aDoença Animal
650 $aPatogenicidade
700 1 $aMANTOVANI, C.
700 1 $aSANTOS, M. G. dos
700 1 $aEGITO, A. A. do
700 1 $aVERBISCK, N. V.
700 1 $aSANTOS, L. R. dos
700 1 $aDÁVILA, A. M. R.
700 1 $aZIMPEL, C. K.
700 1 $aZERPA, M. C. S.
700 1 $aCHIEBAO, D. P.
700 1 $aGUIMARÃES, A. M. DE S.
700 1 $aNASSAR, A. F. DE C.
700 1 $aARAUJO, F. R.
773 $tPathogens$gv. 12, e1250, 2023.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
18/02/2019 |
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Data da última atualização: |
18/02/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
LUIZ, O. J.; WEBER, V. A. de M.; CAGNIN, M. I.; MEDEIROS, S. R. de; GOMES, R. da C.; SOUZA, L. C. de; PAIVA, D. M. B. |
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Afiliação: |
OLAVO J. LUIZ, IFPR; VANESSA APARECIDA DE MORAES WEBER, UCDB and UEMS; MARIA ISTELA CAGNIN, Universidade Federal de Mato Grosso do Sul - UFMS; SERGIO RAPOSO DE MEDEIROS, CNPGC; RODRIGO DA COSTA GOMES, CNPGC; LEILIANE CRISTINE DE SOUZA, IFPR; DÉBORA MARIA BARROSO PAIVA, Universidade Federal de Mato Grosso do Sul - UFMS. |
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Título: |
Computational system to support bovine nutritional behavior. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
International Journal of Agricultural and Environmental Information Systems V, 9, n. 3, p. 22-37, July-September 2018 |
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Idioma: |
Inglês |
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Conteúdo: |
Computational methodologies and techniques are increasingly being used for agricultural purposes in recent years. This article presents a computational system composed of hardware and software elements that can be used in the Precision Livestock area. The objective is to support the management of the operation of providing nutritional supplements: a hardware module identifies the visits of the animals to the troughs and transmits the data to a software module, which processes them and generates reports to support decision-making. A case study indicated the viability of the solution and advantages of the system: portability and flexibility in the expansion of the functionalities through modularization, for less than one tenth of the cost of the current solutions available. |
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Palavras-Chave: |
Automated Trough; Capture Station; NutriComBov; Nutritional Management; Precision Farming. |
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Thesaurus NAL: |
Animal behavior; Beef cattle. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01626naa a2200277 a 4500
001 2106168
005 2019-02-18
008 2018 bl uuuu u00u1 u #d
100 1 $aLUIZ, O. J.
245 $aComputational system to support bovine nutritional behavior.$h[electronic resource]
260 $c2018
520 $aComputational methodologies and techniques are increasingly being used for agricultural purposes in recent years. This article presents a computational system composed of hardware and software elements that can be used in the Precision Livestock area. The objective is to support the management of the operation of providing nutritional supplements: a hardware module identifies the visits of the animals to the troughs and transmits the data to a software module, which processes them and generates reports to support decision-making. A case study indicated the viability of the solution and advantages of the system: portability and flexibility in the expansion of the functionalities through modularization, for less than one tenth of the cost of the current solutions available.
650 $aAnimal behavior
650 $aBeef cattle
653 $aAutomated Trough
653 $aCapture Station
653 $aNutriComBov
653 $aNutritional Management
653 $aPrecision Farming
700 1 $aWEBER, V. A. de M.
700 1 $aCAGNIN, M. I.
700 1 $aMEDEIROS, S. R. de
700 1 $aGOMES, R. da C.
700 1 $aSOUZA, L. C. de
700 1 $aPAIVA, D. M. B.
773 $tInternational Journal of Agricultural and Environmental Information Systems V, 9$gn. 3, p. 22-37, July-September 2018
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